Dataset Open Access
De, Trina; Urbanski, Adrian; Thangamani, Subasini; Wyrzykowska, Maria; Yakimovich, Artur
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="DOI">10.14278/rodare.3001</identifier> <creators> <creator> <creatorName>De, Trina</creatorName> <givenName>Trina</givenName> <familyName>De</familyName> <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-1111-9851</nameIdentifier> <affiliation>Center for Advanced Systems Understanding, Helmholtz-Zentrum Dresden-Rossendorf</affiliation> </creator> <creator> <creatorName>Urbanski, Adrian</creatorName> <givenName>Adrian</givenName> <familyName>Urbanski</familyName> <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0009-0008-6619-3665</nameIdentifier> <affiliation>Center for Advanced Systems Understanding, Helmholtz-Zentrum Dresden-Rossendorf</affiliation> </creator> <creator> <creatorName>Thangamani, Subasini</creatorName> <givenName>Subasini</givenName> <familyName>Thangamani</familyName> <affiliation>Center for Advanced Systems Understanding, Helmholtz-Zentrum Dresden-Rossendorf</affiliation> </creator> <creator> <creatorName>Wyrzykowska, Maria</creatorName> <givenName>Maria</givenName> <familyName>Wyrzykowska</familyName> <affiliation>Center for Advanced Systems Understanding, Helmholtz-Zentrum Dresden-Rossendorf</affiliation> </creator> <creator> <creatorName>Yakimovich, Artur</creatorName> <givenName>Artur</givenName> <familyName>Yakimovich</familyName> <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-2458-4904</nameIdentifier> <affiliation>Center for Advanced Systems Understanding, Helmholtz-Zentrum Dresden-Rossendorf</affiliation> </creator> </creators> <titles> <title>HeLaCytoNuc: fluorescence microscopy dataset with segmentation masks for cell nuclei and cytoplasm</title> </titles> <publisher>Rodare</publisher> <publicationYear>2024</publicationYear> <subjects> <subject>Fluorescence microscopy</subject> <subject>high content microscopy</subject> <subject>cytoskeleton</subject> <subject>cell nuclei</subject> </subjects> <dates> <date dateType="Issued">2024-06-05</date> </dates> <language>en</language> <resourceType resourceTypeGeneral="Dataset"/> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://rodare.hzdr.de/record/3001</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="URL" relationType="IsIdenticalTo">https://www.hzdr.de/publications/Publ-39181</relatedIdentifier> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.14278/rodare.3000</relatedIdentifier> <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://rodare.hzdr.de/communities/health</relatedIdentifier> <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://rodare.hzdr.de/communities/rodare</relatedIdentifier> </relatedIdentifiers> <version>Version 1</version> <rightsList> <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract"><p><strong>Data Description:</strong></p> <p>This dataset comprises fluorescence micrographs of HeLa cells, specifically labelled to identify nuclei and cell cytoplasm. These images were acquired as a technical calibration for a high-content screening study detailed and published in [1].</p> <p>The HeLa cell line (ATCC-CCL-2), a widely used immortalised cell line in laboratory research, was cultured under standard conditions. Post-cultivation, the cells were fixed and stained with fluorescent dyes to visualise the nuclei and cytoplasm. The nuclei were stained with DAPI (4&#39;,6-diamidino-2-phenylindole), a blue-fluorescent DNA stain, while fluorescent-labeled phalloidin was used to detect actin filaments and delineate the cytoplasm. The entire process of cell culture, fixation, staining, and imaging adhered strictly to the protocols described in [1].</p> <p>The preprocessed dataset includes 2,676 8-bit RGB images, each with a pixel resolution of 520 x 696 pixels. In these images, only two of the RGB channels are utilized: the red channel represents the cytoplasm, and the blue channel represents the nuclei. The dataset is divided into training, validation, and test subsets in a 70:20:10 ratio. The entire dataset is accompanied by instance segmentation masks for nuclei and cytoplasm objects obtained through a specialised CellProfiler [2] software. Notably, the test subset was annotated manually by a specialist, ensuring high-quality annotations. The original raw images are of a higher resolution, 1040 x 1392 pixels, and have a bit depth of 16 bits, providing more detailed information for advanced analyses.</p> <p><br> <strong>File&nbsp;Description:</strong></p> <p>The file structure of the zip files is as follows:</p> <p>HeLaCytoNuc_{train/validation/test}.zip -&gt;</p> <p>- images -&gt; {filename}.tif</p> <p>- nuclei_masks&nbsp; -&gt; {filename}.tif</p> <p>- cytoplasm_masks&nbsp; -&gt; {filename}.tif</p> <p>HeLaCytoNuc_raw_images.zip -&gt; {filename}.tif</p> <p>HeLaCytoNuc_test_cellprofiler_masks.zip -&gt;</p> <p>- nuclei_masks&nbsp; -&gt; {filename}.tif</p> <p>- cytoplasm_masks&nbsp; -&gt; {filename}.tif&nbsp;</p> <p><strong>References:</strong></p> <p>1.&nbsp;R&auml;m&ouml;, Pauli, Anna Drewek, C&eacute;cile Arrieumerlou, Niko Beerenwinkel, Houchaima Ben-Tekaya, Bettina Cardel, Alain Casanova et al. &quot;Simultaneous analysis of large-scale RNAi screens for pathogen entry.&quot;&nbsp;<em>BMC genomics</em>&nbsp;15 (2014): 1-18.</p> <p>2.&nbsp;Carpenter, Anne E., Thouis R. Jones, Michael R. Lamprecht, Colin Clarke, In Han Kang, Ola Friman, David A. Guertin et al. &quot;CellProfiler: image analysis software for identifying and quantifying cell phenotypes.&quot;&nbsp;<em>Genome biology</em>&nbsp;7 (2006): 1-11.</p></description> </descriptions> </resource>
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