Plot Open Access

Not just a background: pH buffers do interact with lanthanide ions – a Europium(III) case study

Mandal, Poulami; Kretzschmar, Jérôme; Drobot, Björn

The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry viz. HEPES, PIPES, MES, MOPS, and TRIS have been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good’s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good’s buffers. 

Files (5.5 MB)
Name Size
Manuscript.docx
md5:fca24f697935458f73d84814fa844eb4 		5.5 MB Download
Supporting informations.docx
md5:58bf37346e25132abf9ea8c10655d59f 		16.5 kB Download
609
156
views
downloads
All versions This version
Views 609609
Downloads 156156
Data volume 467.9 MB467.9 MB
Unique views 477477
Unique downloads 141141
More info on how stats are collected.
Publication date:
September 9, 2021
DOI:
10.14278/rodare.1148

RODARE DOI Badge

DOI 

10.14278/rodare.1148

Markdown 

[![DOI](https://rodare.hzdr.de/badge/DOI/10.14278/rodare.1148.svg)](https://doi.org/10.14278/rodare.1148)

reStructedText 

.. image:: https://rodare.hzdr.de/badge/DOI/10.14278/rodare.1148.svg
   :target: https://doi.org/10.14278/rodare.1148

HTML 

<a href="https://doi.org/10.14278/rodare.1148"><img src="https://rodare.hzdr.de/badge/DOI/10.14278/rodare.1148.svg" alt="DOI"></a>

Image URL 

https://rodare.hzdr.de/badge/DOI/10.14278/rodare.1148.svg

Target URL 

https://doi.org/10.14278/rodare.1148

Keyword(s):
Europium Buffer TRLFS NMR Stability Constant
Related identifiers:
Cites:
10.17815/jlsrf-2-58
Identical to:
https://www.hzdr.de/publications/Publ-33100
Referenced by:
https://www.hzdr.de/publications/Publ-33074
Communities:
License (for files):
Creative Commons Attribution 4.0 International

Versions

Version 1 10.14278/rodare.1148 Sep 9, 2021
Cite all versions? You can cite all versions by using the DOI 10.14278/rodare.1147. This DOI represents all versions, and will always resolve to the latest one. Read more.

Share

Cite as

Mandal, Poulami, Kretzschmar, Jérôme, & Drobot, Björn. (2021, September 9). Not just a background: pH buffers do interact with lanthanide ions – a Europium(III) case study. Rodare. http://doi.org/10.14278/rodare.1148
Loading...

Export