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Not just a background: pH buffers do interact with lanthanide ions – a Europium(III) case study
Mandal, Poulami; Kretzschmar, Jérôme; Drobot, Björn
The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry viz. HEPES, PIPES, MES, MOPS, and TRIS have been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good’s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good’s buffers.
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Manuscript.docx
md5:fca24f697935458f73d84814fa844eb4 |
5.5 MB | Download |
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Supporting informations.docx
md5:58bf37346e25132abf9ea8c10655d59f |
16.5 kB | Download |
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- Publication date:
- September 9, 2021
- DOI:
-
- Keyword(s):
- Europium Buffer TRLFS NMR Stability Constant
- Related identifiers:
- Cites:
10.17815/jlsrf-2-58 - Identical to:
https://www.hzdr.de/publications/Publ-33100 - Referenced by:
https://www.hzdr.de/publications/Publ-33074 - Communities:
- License (for files):
- Creative Commons Attribution 4.0 International