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DNA Based Formation of Nanodisc-Stacks
Subramanian, Madhumalar;
Kielar, Charlotte;
Tsushima, Satoru;
Fahmy, Karim;
Oertel, Jana
We describe here the formation of multimers of membrane-scaffolding protein MSP1D1-bounded nanodiscs using the thiol reactivity of engineered cysteines. The mutated positions N42 and K163 in MSP1D1 were chosen to support chemical modification as evidenced by fluorescent labeling with pyrene. The direct disulphide bond formation of nanodiscs formed by the MSP1D1_N42C variant led to dimers and trimers with low yield. In contrast, transmission electron microscopy revealed that the attachment of oligonucleotides to the engineered cysteines of MSP1D1 allowed the growth of submicron-sized tracts of stacked nanodiscs through the hybridization of nanodisc populations carrying complementary strands and a flexible spacer.
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- Publication date:
- December 14, 2021
- DOI:
-
- Keyword(s):
- membrane-scaffolding protein nanodisc membrane protein lipid bilayer lipid protein interaction multimerization self-assembly bionanotechnology
- Related identifiers:
- Identical to:
https://www.hzdr.de/publications/Publ-32927 - Referenced by:
https://www.hzdr.de/publications/Publ-32443 - Communities: