Title:			HPLC Data Explanation		
Investigator:	Max Klotzsche	
Date:			07.01.2025		
Location:		Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany

Experiment:		Xylem Sap Collection 
Methods:		HPLC
Instrument:		Agilent 1200, Nucleogel ION 300 OA, 5 mM H2SO4 @ 0.4 mL/min

Files:			Calibration (20 µL injection volume each)
					8465	Oxalic acid 10 mM
					8466	Oxalic acid 1 mM
					8467	Oxalic acid 0.1 mM
					8468	Citric acid 10 mM
					8469	Citric acid 1 mM
					8470	Citric acid 0.1 mM
					8471	Malic acid 10 mM
					8472	Malic acid 1 mM
					8473	Malic acid 0.1 mM
					8474	Fumaric acid 1 mM
					8475	Fumaric acid 0.1 mM
					8476	Fumaric acid 0.01 mM
				Xylem Sap
					8477	200 µM approach - A1 (90 min)
					8478	200 µM approach - A2 (180 min)
					8479	200 µM approach - A3 (225 min)
					8480	200 µM approach - A4 (270 min)
					8481	200 µM approach - A5 (315 min)
					8482	200 µM approach - A6 (360 min)
					8503	0 µM Control Xylem Sap (90 min)

Description:	Calibration was carried out with three concentrations per organic acid
				Peak areas were used to create a linear regression in order to calculate the organic acid concentrations in xylem sap samples