Title:			Xylem Sap Analysis Data Explanation		
Investigator:	Max Klotzsche	
Date:			07.01.2025		
Location:		Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany

Experiment:		Xylem Sap Collection 
Methods:		HPLC, TRLFS, ICP-MS
Instrument:		HPLC: Agilent 1200, Nucleogel ION 300 OA, 5 mM H2SO4 @ 0.4 mL/min
				TRLFS: Ekspla NT230-50-SH/SF-SCU (394 nm exc. wavelength), Shamrock SR-303i-A spectrograph, iStar DH320T-18U-63 ICCD 
				ICP-MS: NexION 350x, Ar plasm
Files:			Origin File
					Folder HPLC contains:					-chromatograms of xylem sap samples A1...A5
															-chromatograms of citric, malic, fumaric and oxalic acid
															-concentration curves of these organic acids in the samples A1...A5
															-chromatogram of xylem sap sample from a plant which was not exposed to Eu(III) (Control, 0µM)
					Folder HPLC Calibration Data contains: 	-chromatograms of the aforementioned organic acids, three different concentrations each
					Folder HPLC References contains:		-chromatograms of all tested organic acids
Description:		Folder ICP-MS Data contains:			-raw data (concentration) of Eu(III) in various xylem sap samples
															-mean values of three biological replicates for each approach (200, 20,2 µM Eu(III) in medium)
															-respective graphs
					Folder ICP-MS Data time-delayed contains: -data from separate experiment in which all plants were exposed to 200 µM, but the shoot was cut at different times after initial root exposure
					Folder TRLFS contains:					-luminescence emission data of xylem sap samples A2...A6, measured in a microcuvette
															-luminescence emission data of artificially mixed xylem sap sample, measure in a microcuvette
															-respective graphs