Title:			Autoradiography Explanation		
Investigator:	Max Klotzsche	
Date:			07.01.2025		
Location:		Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany

Experiment:		Autoradiography with 152Eu(III)-afflicted A. strigosa plants 
Methods:		Autoradiography, Image processing
Instruments:	Multipurpose storage phosphor screen (20x40 cm2), Bio-Rad Pharos FX biomolecular imager and Quantity One (software), ImageJ
Files:			Folder "Raw_Autoradiograms"
					-contains Quantity One internal file format .1sc of autoradiograms
					-contains autoradiograms in .tif file format
				Folder "Calibrated_Autoradiograms_Root"	
					-contains root autoradiograms which were calibrated using Image J in three different representations: black and white, 16 colors and so-called 'fire'
					-each representation is saves as .tif and .png
				Folder "Calibrated_Autoradiograms_Shoot"
					-contains respective shoot autoradiograms
				Excel file "AR_Calibration_Root"/"AR_Calibration_Shoot" 
					-was used to correlate the integrated density of the gray values of the internal calibration standards with known 152Eu(III) activities and hence its amount of substance
					-a linear regression curve was then used to calibrate the autoradiogram in ImageJ